PLANT TISSUE CULTURE WORKSHOP FOR STUDENTS OF SENIOR HIGH SCHOOL 7 YOGYAKARTA

Authors

  • (*) Ratih Restiani None,  Faculty of Biotechnology, Duta Wacana Christian University
  • Catarina Aprilia Ariestanti,  Prodi Biologi, Fakultas Bioteknologi, Universitas Kristen Duta Wacana, Yogyakarta
  • Laurentia Henrieta Permita Sari Purba,  Prodi Biologi, Fakultas Bioteknologi, Universitas Kristen Duta Wacana, Yogyakarta
  • Theresia Sri Retnowati,  Prodi Biologi, Fakultas Bioteknologi, Universitas Kristen Duta Wacana, Yogyakarta
  • Josiah Herald Matheos,  Prodi Biologi, Fakultas Bioteknologi, Universitas Kristen Duta Wacana, Yogyakarta
  • Sarah Mega Pratenna Kaban,  Prodi Biologi, Fakultas Bioteknologi, Universitas Kristen Duta Wacana, Yogyakarta
  • Astrid Ayu Sekar,  Prodi Biologi, Fakultas Bioteknologi, Universitas Kristen Duta Wacana, Yogyakarta

(*) Corresponding Author

DOI:

https://doi.org/10.21460/servirisma.2023.32.44

Keywords:

biotechnology, community service, plant tissue culture, senior high school, workshop

Abstract

Plant tissue culture is one of the topics in the Biotechnology module of Biology for senior high school class XII. This topic has not been optimally provided in schools, especially in SMA Negeri 7 Yogyakarta due to the limited human resources who are experienced in plant tissue culture and the unavailability of facilities and equipment that support the implementation of the practicum topic. Therefore, this workshop aimed to help students gain knowledge and skills regarding the basic principles, benefits, and stages of plant tissue culture. The workshop was conducted in the Biology Laboratory of SMA Negeri 7 Yogyakarta and participated by 10 class XII students. The workshop consisted of questions and answers before the workshop, materials explanation, hands-on activities, questions and answers after the workshop, and evaluation. The material was delivered using lecturing methods and interactive discussions, followed by practical activities including preparation of culture media, sterilization and inoculation of explants and acclimatization which were carried out by engaging the active participation of each student. The results obtained in this workshop were that students were able to understand and implement plant tissue culture practical activities as well.

 

References

Abdalla, N., El-Ramady, H., Seliem, M. K., El-Mahrouk, M. E., Taha, N., Bayoumi, Y., Shalaby, T. A., & Dobránszki, J. (2022). An Academic and Technical Overview on Plant Micropropagation Challenges. Horticulturae, 8(8). https://doi.org/10.3390/horticulturae8080677

Bhojwani, S. S., & Dantu, P. K. (2013). Plant tissue culture: An introductory text. Plant Tissue Culture: An Introductory Text, 1–309. https://doi.org/10.1007/978-81-322-1026-9

Custódio, L., Charles, G., Magné, C., Barba-Espín, G., Piqueras, A., Hernández, J. A., Ben Hamed, K., Castañeda-Loaiza, V., Fernandes, E., & Rodrigues, M. J. (2023). Application of In Vitro Plant Tissue Culture Techniques to Halophyte Species: A Review. Plants, 12(1), 1–34. https://doi.org/10.3390/plants12010126

Kasim, H., Basri, Z., Amir, J., & Nursalam. (2020). Pelatihan Kultur Jaringan Kepada SMA dan SMK. Mosintuvu: Jurnal Pengabdian Kepada Masyarakat, 1(Desember 2020), 63–70. http://jurnal.faperta.untad.ac.id/index.php/MOSINTUVU/article/view/632/622

Kementerian Pendidikan dan Kebudayaan Direktorat Jenderal Pendidikan. (2020). Biologi Kelas XII - KD 3.1. 1–48.

Kurnianingsih, R., Ghazali, M., Rosidah, S., Muspiah, A., Astuti, S. ., & Nikmatullah, A. (2020). Pelatihan Teknik Dasar Kultur Jaringan Tumbuhan. JMM (Jurnal Masyarakat Mandiri), 4(5), 888–896. http://journal.ummat.ac.id/index.php/jmm/article/view/3049

Moda, K. F. (2022). Pelatihan Kultur Jaringan sebagai Upaya Peningkatan Keterampilan di SMA Negeri 1 Glagah. 20180308022.

Nurcahyani, E., Zulkifli, Z., & Kanedi, M. (2021). Pengenalan dan Pelatihan Teknik Kultur Jaringan Tumbuhan Bagi Guru Biologi SMA Se-Kabupaten Tanggamus Provinsi Lampung. Jurnal Pengabdian Kepada Masyarakat (JPKM) TABIKPUN, 2(1), 39–46. https://doi.org/10.23960/jpkmt.v2i1.26

Restiani, R. (2022). Pelatihan Kultur Jaringan Anggrek Sebagai Upaya Peningkatan Keterampilan Bioteknologi di SMA Santa Maria Yogyakarta. Sendimas. https://sendimas2022.maranatha.edu/index.php/2022/2022/paper/viewFile/190/110

Restiani, R., Dolonseda, A. C., Kaban, S. M. P., Hutabarat, C. T., Sekar, A. A., Meliana, F. A., Linardi, M., Verrell, N., & KY, A. A. B. (2022). Efficient Callus and Shoot Induction Protocol from Leaf and Node Explants of Javanese Ginseng (Talinum paniculatum (Jacq.) Gaertn.). Scholars Journal of Agriculture and Veterinary Sciences, 9(12), 223–231. https://doi.org/10.36347/sjavs.2022.v09i12.003

Sharma, H., & Vashistha, B. D. (2015). Plant tissue culture: a biological tool for solving the problem of propagation of medicinally important woody plants-A review. International Journal of Advanced Research, 3(2), 402–411.

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Published

2023-11-30

How to Cite

None, R. R., Ariestanti, C. A., Purba, L. H. P. S., Retnowati, T. S., Matheos, J. H., Kaban, S. M. P., & Sekar, A. A. (2023). PLANT TISSUE CULTURE WORKSHOP FOR STUDENTS OF SENIOR HIGH SCHOOL 7 YOGYAKARTA. Servirisma, 3(2), 81–90. https://doi.org/10.21460/servirisma.2023.32.44